High Performance Thin Layer Chromatograph (HPTLC), (Camag, Switzerland)
HPTLC is the most simple separation technique today available to the analyst. It can be considered a time machine that can speed your work and allows you to do many things at a time usually not possible with other analytical techniques.
Principle
HPTC take place in high speed capillary flow rang of the mobile phase. There are three main steps in HPT C
1] sample to analyzed to chromatogram layer volume precision and suitable position are achieved by use of suitable instrument.
2]solvent (mobile phase) migrates the planned distance in layer(stationary) by capillary action in this process sample separated in its components.
3] separation tracks are scanned in densitometer with light beam in visible or uv region.
